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Determining the Optimum pH for Trypsin - Biocyclopedia
- https://biocyclopedia.com/index/biotechnology_methods/enzymology/determining_the_optimum_ph_for_trypsin.php#:~:text=Add%20a%20strip%20of%20photographic%20film%20into%20the,trypsin%20solution%20into%20a%20test%20tube%20with%20film.
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To investigate the effect of Trypsin using a photographic film
- https://blablawriting.net/prime-investigate-effect-trypsin-using-photographic-film-essay
- Preliminary experiment: We are investigating the best pH at which Trypsin works. Method: A beaker was filled with water of about 400C. Each test tube was filled with 5cm3 of trypsin and 5cm3 of water with a buffer solution one pH 4 one pH 7 and the other pH 9. A piece of 1cm2 photographic film was dropped in. At this time the stop clock was started.
To investigate the effect of Trypsin using a photographic film
- https://www.markedbyteachers.com/as-and-a-level/science/to-investigate-the-effect-of-trypsin-using-a-photographic-film.html
- A piece of the film was placed in each test tube. At this time a stop clock is started. Each test tube is monitored until the film goes clear. Results table: Analysis: The optimum pH of the enzyme was pH 9, which is what I predicted the preliminary experiment showed this.
Investigating effect of concentration on the activity of …
- https://practicalbiology.org/bio-molecules/factors-affecting-enzyme-activity/investigating-effect-of-concentration-on-the-activity-of-trypsin
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Determining the Optimum pH for Trypsin - Biocyclopedia
- https://biocyclopedia.com/index/biotechnology_methods/enzymology/determining_the_optimum_ph_for_trypsin.php
- Procedure Set up the water bath and adjust the temperature to 37°C. Pipette 1 mL buffer solution of pH 1.0 into a test tube. Add a strip of photographic film into the solution and put it into the water bath for 5 minutes. Pipette 1 mL of trypsin solution into another test tube. Put it into the water bath for 5 minutes.
Trypsin and pH - YouTube
- https://www.youtube.com/watch?v=yyGJgUGxut0
- Shows digestion of gelatin on photographic film by trypsin at a range of pH's.
Procedure - Royal Society of Biology
- https://practicalbiology.org/images/pbiol/doc/migrated/investigating-effect-of-concentration-on-the-activity-of-trypsin-ss-33__1_.docx
- Place the test tubes and the thermometer in a beaker of warm water at close to 35ºC. Maintain the temperature within two degrees of this during the course of the investigation by adding more hot water as necessary. Make a note of the temperature of the water. Cut pieces of photographic film to fit in the test tube as shown.
To investigate the effect of temperature on the rate of reaction on ...
- https://blablawriting.net/prime-investigate-effect-temperature-rate-reaction-trypsin-enzymes-essay
- Carefully place a 1cm piece of photographic film in each notch on the splints. 8. Place three test tubes in the water bath. 9. Set the thermostat on the water bath to 10C. 10. Wait 10-12 minutes for the water to settle at 10C. 11. Place a splint with photographic film into each of the test tubes. 12. Start timing using the stopwatch. 13.
Biology Coursework: The effect of Trypsin on Gelatine. | 123 Help …
- https://www.123helpme.com/essay/Biology-Coursework-The-effect-of-Trypsin-on-55569
- § Photographic film (b/w) § Syringe § Test tube rack § Ruler § Scissors § O.1% trypsin made in pH7 buffer solution § Mounted needle 2. I then decided on the range of results and temperatures I was to investigate. I decided to observe 20º, 30º, 40º, 50º, 60º, 70º and 80º. 3.
Investigating The Rate Of Reaction Of Enzymes In The Clearing Of ...
- https://essaymania.com/50508/investigating-the-rate-of-reaction-of-enzymes-in-the-clearing-of-photographic-film
- In the investigation I am going to study one of the four factors that alter the rate of reaction of trypsin in the clearing of photographic film. In photographic film is the clear acetate at the bottom and on top of that is geletin (a substrate) which has silver salts in it. When trypsin is added, the geletin is broken down and the acetate becomes clear. This can occur at different …
The Film Lab: 10 Analogue Experiments - Lomography
- https://www.lomography.com/magazine/320169-the-film-lab-10-analogue-experiments
- Just drench your film rolls in black coffee and vitamin C to get those rich hues of dark brown and black. Best films to use are Ilford HP5 Plus 400 ISO, Kodak TMAX 400, Ilford FP4 ( ISO 125), and Ilford PanF ( ISO 50). Credits: crossbrasil, alexyz & missnothing. Learn how to do the caffenol process in the article Alternative Processes: Caffenol.
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